Scientists from North Carolina State University has been able to make designer biosensors which can detect the antibiotic molecules of interest within microbes like E.coli.
Macrolides are considered to be a group of naturally generated small molecules which have antibiotic, antifungal and anticancer effects. For example, antibiotic erythromycin is a macrolide which is produced by the soil-dwelling bacteria.
Though the microbes that produce antibiotic macrolide are only a small amount of a limited variety of antibiotics yet the scientist are interested to use them for developing new antibiotics.
As per Gavin Williams the Associate Professor of Bio-organic Chemistry at NC State University, the aim of the research team is to engineer new microbes to create new versions of antibiotics for people, which can drastically reduce the cost and time to make a new one and test it.
The report of his research has been published in ACS Synthetic Biology. The overall research has been funded by the National Institutes of Health and the NC State Chancellor’s Innovation Fund. In order to do that, they need to detect the antibiotic molecules of interest within the microbes.
For this experiment, Williams and his team used a protein called MphR as a natural switch. The MphR is able to detect the presence of macrolide antibiotic in E.coli and starts a resistive mechanism to defy the effects of antibiotics.
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Huge collection of MphR
Based on this mechanism the scientists have created a huge collection of MphR protein variants based on their ability on the production of a fluorescent green protein component when they are in the presence of a necessary macrolide.
Williams and his team have successfully done the test macrolides like erythromycin where the MphR already recognized it and some of the MphR variants even increased their ability to detect like ten times than a normal case.
The team has also successfully done the test with antibiotics like tylosin which is not related to erythromycin in any way.
As per Williams, they have engineered the MphR sensor system by increasing the sensitivity of recognizing the antibiotic molecules of interest which will help in screening many more different antibiotics easily and quickly.
As per him, this is their first step towards a high end engineered antibiotics which can be generated from a huge collection of genetically modified strains and microbes. From there they can get the selected variants of strains to make the desired antibiotic molecule.
According to the scientists, this research can reshape the world of antibiotics in near future, when we can depend on natural antibiotics.